AbClon

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Epitope Screening Service

Epitope Screening

Antibody in the serum and purified antibody binds to which part of the antigen protein. Based on PEPper-
PRINT's proprietary PEPperCHIP platform technology, it prints amino acids directly on slides using amino acid
printers to produce peptide arrays; instead it synthesizes existing peptides and binds them to slides.

Features

  • Amino acid is synthesized as a microparticle in the form of peptide synthesis by print
  • High density / high content Spot (1,000 peptides / cm2)
  • Non-specific protein interactions are prevented to minimize background
  • Duplicate spot generation and repeated testing on the same peptide maximize confidence in results
  • Multi-array with one slide

Epitope screening type

  • Linear epitope screening
  • Conformational epitope screening
  • Epitope substitution screening
  • Research Screening
  • Discovery Screening

Customer Preparation

  • Protein sequence
    • - The sequence to create an array (Ligand)
  • Analyte
    • - Antibodies, serum, ascites, and proteins
    • - antibody requirement
      • · Linear epitope mapping : 20 ~ 30 ug (serum : 20 ~ 40 ul)
      • · Conformational epitope mapping : 100 ~ 200 ug (Serum : 200 ~ 400 ul)

Applications

  • Antibody analysis
    • - Epitope mapping, cross-reactivity analysis
  • Cancer Research
    • - Analysis of specific tumor antibody response through serum
    • - Monitor cancer-related antibody response
  • Vaccine development
    • - immune monitoring
    • - HLA Class Screening
  • Infectious disease
    • - Identification of biomarkers

Linear Epitope mapping

Conformational Epitope mapping

  • goal : epitope mapping of Rituximab (Rituxan), a chimeric monoclonal anti-CD20 antibody
  • microarray : CD20 as linear and cyclic constrained overlapping peptides (7 aa, 10 aa and 13 aa)
  • sample : monoclonal antibody Rituximab
  • outcome : high resolution data with EPANPSEK as Rituximab epitope


문의사항

+82-2-2109-1294