AbClon

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Antibody Production Purification Service

In vitro / In vivo antibody production

AbClon provide the mass production service of monoclonal antibody using existing hybridoma
or hybridoma produced by AbClon.

Type & volume

Type & volume
Kinds Volume
In vitro production 100ml culture ~
In vivo production 5 mice ~

Features

  • In vitro benefits
    • - Possible to eliminate contamination of other antibodies, for in vitro assay
  • In vivo benefits
    • - A large amount of antibodies can be obtained, for development of diagnostic kits
  • Experiment progress data transmission and 1: 1 customized consultation service
  • Continuous delivery of antibodies of the same quality
  • Antibodies with one epitope are highly specific

Customer Preparation

  • Hybridoma cell
    • - Hybridoma cell stock
    • - Cell and culture information
    • - Production conditions
    • - Antigen (for antibody detection)

Related Service

  • Antibody purification service
    • - IgG purification: Whole IgG purification in serum or ascites using protein A, G
    • - Affinity purification: specific IgG binding to the antigen is purified
  • Epitope mapping
    • - Identification of epitope binding to target using ascites, media or purified antibody
  • Antibody conjugation
    • - Biotin
    • - HRP (Horseradish peroxidase)
    • - FITC (Fluorescein isothiocyanate)
    • - PE (Phycoerythrin)

Service Flow

In vitro production
  • Hybridoma
    Cell thawing & Culture
    • · Cell status check
    • · One week
  • 1st Cell expansion
    • · 1~2 weeks
  • 2nd Cell expansion
    • · 1~2 weeks
  • Culture Supernatant
    Harvast & QC
    • · SDS-PAGE
      confirmation
    • · One week
  • Delivery or
    Next process
    • · complete
In vivo production
  • Mouse Adaptation
    • · Mouse Stabilization
    • · 2 weeks
  • Pristane Injection
    • · One week
  • Hybridoma
    cell Injection
    • · One week
  • Ascites Isolation and QC
    • · SDS-PAGE
      confirmation
    • · 2 weeks
  • Delivery or
    Next process
    • · complete
    • · report

IgG / Affinity antibody purification

AbClon provide the antibody purification service from ascites, cultured media or serum.

Kind and base unit

Kind and base unit
Type Ligand Base unit
IgG purification Protein A, G Ascites or serum: 10 ml
cultured media: 100 ml
Affinity Tablets Protein or peptide

Features

  • Advantages of IgG purification
    • - High amount of purified antibody
  • Affinity Tablet Advantage
    • - Highly specific antibodies can be obtained
    • - Modification antibody can be purified

Customer Preparation

  • Serum, ascites, cultured media, etc.
  • Requires Ligand for Affinity Purification (Protein or Peptide)

Related Service

  • Epitope mapping
    • - Identification of epitope binding to target using ascites, media or purified antibody
  • Antibody conjugation
    • - Biotin
    • - HRP (Horseradish peroxidase)
    • - FITC (Fluorescein isothiocyanate)
    • - PE (Phycoerythrin)

Service Flow

IgG purification
  • IgG purification
    • · Protein A & G
    • · One week
  • Concentration & QC
    • · Concentration
    • · Quantitative & SDS-PAGE
    • · One week
  • Delivery or Next process
    • · report
Affinity Tablets
  • Ligand confirmation &
    IgG purification
    • · Protein A & G
    • · One week
  • Affinity purification
    • · Affi-Gel
    • · One week
  • Concentration & QC
    • · Concentration
    • · Quantitative & SDS-PAGE
    • · One week
  • Delivery or Next process
    • · report

문의사항

+82-2-2109-1294