AbClon

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Antibody engineering services

Antibody Sequencing

Identification of antibody gene sequence information is an essential process for functional opti-
mization, database banking and patent applications antibody engineering, such as humanization and
affinity maturation of monoclonal antibodies (mAbs) . It is a service that can strongly recom-
mend even if cell line management is difficult after Hybridoma development. Hybridoma can
contain pseudogenes and mRNA encoding non-specific antibodies, which can be amplified by
primers to clone the target antibody, after sequencing 3 to 5 clones independent of the
antibody, it is produced in the form of a Fab and reacts with the antigen by ELISA to ensure
accuracy.

Features

  • Identify variable region sequences that react with antigens by Fab construction
  • Duration: about 6 weeks
  • Rich experience in mAb sequencing

Customer Preparation

  • Hybridoma cell (>1×106 cells)
  • Antigen (50 ug)

AbClon provided

  • Variable region sequence
  • Variable region DNA (H-L chain)

Service Flow

Antibody humanization

Non-hHuman monoclonal antibody can beis more than 90% humanized while maintaining affinity and activity. The CDR grafting platform technology, which has know-how in the development of antibodies in AbClon, is accurate and reliable.

Features

  • Reliable CDR grafting platform
  • Duration: about 3 months
  • Technology development for therapeutic antibody

Customer Preparation

  • Variable region sequence
  • The antigen (500 ug)
  • Backbone (optional)

AbClon provided

  • Humanized sequence
  • Variable region Humanized DNA (HL chain)
  • Affinity result
  • Full IgG (100 ug)

Service Flow

  • Sequence analysis
    • · Variable region
      analysis
    • · Frame work
      comparison
  • Frame work design
    • · CDR grafting
      platform
  • CDR grafting
    • · Human frame work
  • Humanized
    antibody production
    • · codon optimization
    • · mammalian
      expression
  • Binding affinity
    measurement
    • · octet

Affinity maturation

The affinity of antibody can be increased by engineered affinity maturation process through mutation and selection using antibody library screening technology in-vitro whereas natural affinity maturation can be occurred in vivo by somatic hypermutation and clonal selection. AbClon has developed a phage display library with diversity of antibody variable regions for the purpose of enhancing the binding affinity of antibodies to antigens and develop antibodies with improved affinity.
After measuring the affinity of the original antibody, randomization of the amino acids of the remaining CDRs except for the important amino acid of the antibody proceeds, and Fab type antibody library is developed and an antibody having improved affinity through bio-panning. The diversity of the antibody library developed for affinity maturation is approximately 109 and affinity analysis using Octet confirms affinity enhancement.

Customer Preparation

  • Variable region sequence
  • The antigen (1 mg)
  • DNA template

Offering an app clone (TOP 5 clones)

  • Affinity matured sequence
  • Affinity matured variable region DNA (HL chain)
  • About Binding activity and affinity
  • Fab (100 ug)

Service Flow

Building a Library
  • CDR analysis
    • · Check Affinity effective
  • Library Design
    • · Non-effective
      randomization
  • Fab construction
    • · Antibody library
  • Verify library
    • · Analysis of sequence
Affinity Selection
  • Target binder and
    sequence identification
    • · Panning
    • · ELSIA
    • · Sequencing
  • Affinity measurement
    • · Octet
    • · K-Off ranking TOP 5
  • Fab production
    • · 100ug
    • · TOP 5
  • Fab production
    • · Octet

문의사항

+82-2-2109-1294